Coding

Part:BBa_K4367007:Design

Designed by: August Qvist   Group: iGEM22_Chalmers-Gothenburg   (2022-08-10)


ZF_TM_NubG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 838
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The NubG is a version of the nub part of ubiquitin that has a point mutation.


Source

The ZF transmembrane NubG complex was order for synthesis. Each domain of the complex comes from different sources.

References